Oxyresveratrol- Skin Lightening Potential
Skin Lightening Potential
1. Cell‑based Melanogenesis Inhibitory Assay:
Intracellular melanin in B16F1 melanoma cell lines was treated with various concentrations of Oxyresveratrol over a period of 9 days. melanocyte‑inducing hormone MSH was used to induce melanin in cell lines.
Result : Oxyresveratrol showed a strong inhibitory activity on melanogenesis with IC₅₀ of 1.2 micro gm/ml when compared with Kojic acid IC₅₀ 40.1 µg/mL) and resveratrol 54.6 µg/mL. Oxyresveratrol showed a dose‑dependent inhibitory effect on L-tyrosinase Oxidation. Oxyresveratrol is an inhibitor of tyrosinase activity, but does not suppress the synthesis of tyrosinase. The inhibitory activity is 32-fold more than Kojic acid.
Dose-dependent inhibitory effects on mushroom tyrosinase by oxyresveratrol, resveratrol and kojic acid. : Samples shown are oxy (circle), resveratrol (rectangle)
And kojic acid ( triangle) Effects on tyrosinase activity by samples as a function of concentration are represented as inhibition %, means + – S.E of three independent tests. [ Ref: J of biological chemistry 277, 18, 2002]
2. In vivo evaluation of skin whitening activity of oxyresveratrol solution in volunteers :
A protocol approved clinical study was conducted involved sixty female volunteers. A solution of 0.25% oxyresveratrol (as A.L. Extract), 0.25% Licorice extract and 3% Kojic acid in propylene glycol was used. Application continued for 12 weeks and the melanin content measured at 2‑week. The average percentage whitening increased with treatment duration. 0.18% at 2 weeks to 2.78% at 12 weeks. The results with K.A and W.E during the same period were much lower.
Whitening effect of Oxyresveratrol [ Artocarpus Lakoocha extract] in propylene Glycol in comparison with licorice extract and Kojic acid .Significantly different from control within group, p<0.05 by Student’s t‑test ( adapted from Tanguay et al )
3. In vivo evaluation of lotion containing Oxyresveratrol:
Another study with human volunteers applied a lotion of Oxyresveratrol prepared by oil‑in‑water emulsion and compared with a similar emulsion from Licorice extract . The results were very similar to that of the solution, Oxyresveratrol was the most effective agent, giving the shortest onset of significant whitening effect after only 4 weeks of application, followed 3% KA and 0.25% In Extract. The effect also increased with time with maximum whitening observed at 12 weeks for oxyresveratrol.
Whitening efficacy of Oxyresveratrol [ A lakoocha ] lotion in comparison with licorice lotion. Significantly different from control within group , p <0.05 by Student’s t- test
4. U.V.B. Protection Activity :
Fibroblast cells were treated with varying concentrations of Oxyresveratrol (A.L. Extract) and exposed to U.V.B. dosage. Cells were then incubated and natural red staining technique was used to analyze cell viability. Percentage of U>V. protection was calculated by comparing cytotoxicity in exposed and unexposed cells.
Oxyresveratrol showed 50% reduction in U.V.B. induced cytotoxicity at concentration of 18 µg/mL.
5. Antioxidant potential as measured by ORAC Assay:
ORAC assay is a measure of antioxidant capacity against free radicals. Different concentrations of test sample (oxyresveratrol) were compared with standard compound ———-. The results obtained should a higher ORAC value for oxyresveratrol.
6. Skin anti‑aging potential – Measured by anti‑glycation activity:
Glycation is the reaction that occurs when the reducing portion of monosaccharides such as glucose or fructose ———– to macromolecules of portion or lipid structure to form advanced glycation end products (AGEs), AGEs are related to the pathogenesis of skin aging. Oxyresveratrol exhibited anti glycation with IC₅₀ value of 3.3 µg/mL. Compared to aminoguanidine IC₅₀ 12.5 µg/mL. The ———- antiglycation activity contribute to skin anti aging in formulations.
7. Irritation tests :
Oxyresveratrol does not show skin irritation, Eye irritation, skin sensitization and acute oral toxicity for animals and human skin irritation.
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