Intracellular melanin in B16F1 melanoma cell lines were treated with various concentrations of Oxyresveratrol over a period of 9 days. Melanocyte inducing hormone MSH was used to induce melanin in cell lines.

Result : Oxyresveratrol showed a strong inhibitory activity on melanogenesis with IC50 of 1.2 micro gm/ml when compared with Kojic acid (IC50 40.1 micro gm/ ml) and resveratrol 54.6 micro gm/ ml. Oxyresveratrol showed a dose dependent inhibitory effect on L-tyrosinase Oxidation. Oxyresveratrol is an inhibitor of tyrosinase activity, but dos not suppress the synthesis of tyrosinase. The inhibitory activity is 32 fold more than Kojic acid.

Dose dependent inhibitory effects on mushroom tyrosinase by oxyresveratrol, resveratrol and kojic acid. : Samples shown are oxy (circle), resveratrol (rectangle)

And kojic acid ( triangle) Effects on tyrosinase activity by samples as a function of concentration are represented as inhibition %, means + - S.E of three independent tests. [ Ref: J of biological chemistry 277, 18, 2002]

A protocol approved chemical study conducted involved sixty female volunteers. A solution of 0.25% oxyresveratrol (As A.L. Extract), 0.25% Licorice extract and 3% Kojic acid in propylene glycol were used. Application continued for 12 weeks and the melanin content measured at 2 weeks interval. The average percentage whitening increased with treatment duration,. 0.18% at 2 weeks to 2.78% at 12 weeks. The results with K.A and W.E during the same period were much lower.

Whitening effect of Oxyresveratrol [ Artocarpus Lakoocha extract] in propylene Glycol in comparison with licorice extract and Kojic acid .Significantly different from control within group, P <0.05 by Student's T –test ( adapted from Tanguay et al )

Another study with human volunteers applied a lotion of Oxyresveratrol prepared by oil in water emulsion and compared with a similar emulsion from Licorice extract . The results were very similar to that of the solution Oxyresveratrol was the most effective agent, giving the shortest onset of significant whitening effect after only 4 weeks of application, followed 3% KA and 0.25% In Extract. Effect also increased with time with maximum whitening observed at 12 weeks for oxyresveratrol.

Whitening efficacy of Oxyresveratrol [ A lakoocha ] lotion in comparison with licorice lotion. Significantly different from control within group , P <0.05 by Student's T- test

Fibroblast cells were treated with varying concentration of Oxyresveratrol (A.L. Extract) and exposed to U.V. B dosage. Cells were then incubated and natural red staining technique was used to analyze cell viability. Percentage of U>V. protection was calculated by comparing cytotoxicity in exposed and unexposed cells.

Oxyresveratrol showed 50% reduction in U.V.B induced cytotoxicity at concentration of 18 Mg/ml.

ORAC assay is a measure of antioxidant capacity against free radicals. Different concentration of test sample (oxyresveratrol) were compared with standard compound ----------. The results obtained should a higher ORAC value for oxyresveratrol.

Glycation is the reaction that occurs when the reducing portion of monosaccharides such as glucose or fructose ----------- to macromolecules of portion or lipid structure to form advanced glycation end products (AGE’s), AGE’s are related to the pathogenesis of skin aging. Oxyresveratrol exhibited anti glycation with IC50 value of 3.3 Mg/ml. Compared to aminoguanidine IC50 12.5 Mg/ml. The ---------- antiglycation activity contribute to skin anti aging in formulations.

Oxyresveratrol do not show skin irritation, Eye irritation, skin sensitization and acute oral toxicity for animals and human skin irritation.