Tyrosinase inhibitory activity
A comparative study of Tyrosinase activity
| Compound IC50(muM) | % inhibition(at100muM) | IC50(muM) |
|---|---|---|
|
Kojic acid 133.4 |
41.1 |
133.4 |
|
Licorice extract |
71 |
62 |
|
Oxyresveratrol 12.7 |
83.5 |
12.7 |
|
Dihydro Oxyresveratrol 1.6 |
96.3 |
1.6 |
Dose dependent inhibitory effect on tyrosinase by Dihydro Oxyresveratrol, Oxyresveratrol and Kojic acid.
Samples shown are Oxyresveratrol (Circle), Tetra hydro oxy bibenzyl (triangle) and Kojic acid (diamond). Effects on tyrosinase activity by the samples as a function of concentration are represented as inhibition % , means ± S.E of three independent tests.
Kinetic studies conducted on Dihydro Oxyresveratrol in comparison with oxyresveratrol to measure the ability to inhibit mushroom tyrosinase with L’Dopa as substrate,.
Data were obtained as mean values of I/V, inverse of the increase of absorbance at the wave length 490 mm per minute (∆A490/ min) , with different concentrations of L Dopa as substrate. Inhibitors of the enzyme were tetrahydro bibenzyl with 2.4 Mm( triangle ∆) , 1.2 Mm ( circle O) and No bibenzyl ( circle O)
The observed Km value and V max establish the product as a non competitive inhibitor of tyrosinase on L Dopa. The Ki value obtained in the study establish the compound Dihydro Oxyresveratrol has 5 fold higher affinity to the enzyme than oxyresveratrol.
Before
After
